Effect of Nomolt on Differential and Total Haemocytes in the Desert Locust Schistocerca gregaria Forskal (Orthoptera:Acrididae)

The insecticidal action of Nomolt at various dosages against Schistocerca gregaria was employed for its effects on total haemocyte count (THC)and differential haemocyte count (DHC). The total counts after application of different concentrations from Nomolt were significantly decreased at 1,3, 5 days on 5 nymphal instar and up to 1day old adult males and females comparing with those of the untreated. Changes in DHC have been assessed in relation to application of graded concentrations of Nomolt. The haemocyte profile was determined 1 day, 3 days, 5 days posttreatment as well as 1day in the adult males and females. Different types of haemocytes registered a dose-dependent response by either exhibiting increase or decrease in their relative proportions. The percentage of prohaemocytes, oenocytoids, plasmatocytes, and granulocytes of increased in the treated blood smears as compared to the control while spherulocytes was decreased in the percentage of these cells in treated blood smears. Spherulocytes was the most sensitive cells to the Nomolt whereas the oenocytoids showed least affected cells. However, there was a consistent increase in the proportion of disintegrated cells in accordance with increase in concentration of Nomolt applied. Extreme pathological symptoms were observed in cell membrane, distortion of the cytoplasmic and nuclear membrane, and abnormal staining of the haemocytes. [Salam S. Teleb. Effect of Nomolt on Differential and Total Haemocytes in the Desert Locust Schistocerca gregaria Forskal (Orthoptera:Acrididae). J Am Sci 2011;7(11):479-484]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 60